Measure all the perimeters of your cube and calculate the average.
Calculate your new extrusion multiplier using the formula on the second image. Here is an example:
Your original extrusion multiplier = 1.00 and your average perimeter thickness = 0.441mm.
Your are using a 0.4mm nozzle, so your target/expected extrusion width is 0.45mm.
Your new extrusion multiplier = (1.00 · 0.45)/0.441 = 1.02.
Enter the new extrusion multiplier value in the filament settings page of PrusaSlicer.
Print the part again to verify the value is correct. Repeat the previous steps if necessary.
Tip: The extrusion multiplier is generally constant for a whole spool (if you do not change temperature) but the filament diameter will change. Therefore, before slicing a part, measure the filament diameter you are going to use and enter it into PrusaSlicer.
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